BIOCHEMICAL AND ULTRASTRUCTURAL PROPERTIES OF OSMOTICALLY LYSED RAT-LIVER MITOCHONDRIA

Biochemical and Ultrastructural Properties of Osmotically Lysed Rat-liver Mitochondria

Isolated rat-liver mitochondria were osmotically lysed by suspension and washing 3 times in cold, distilled water. Pellets obtained by centrifugation at 105,000 g for 30 min were resuspended, fixed with glutaraldehyde and OsO(4), and embedded in Epon 812. Thin sections show the presence of two distinct membranous populations, each of which is relatively homogeneous in size and appearance. Swoll...

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OSMOTICALLY LYSED RAT LIVER MITOCtIONDRIA Biochemical and Ultrastructural Properties in Relation to Massive Ion Accumulation

Osmotically lyscd rat liver mitochondria have bccn utilized for a study of the biochemical and ultrastructural properties in relation to divalent ion accumulation. Osmotic lysis of mitochondria by suspension and washing in cold, distilled water results in the extraction of about 50 % of the mitochondrial protein, the loss of the outer mitochondrial membrane,, an increase in respiration, and a m...

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OSMOTICALLY LYSED RAT LIVER MITOCtIONDRIA Biochemical and Ultrastructural Properties in Relation to Massive Ion Accumulation

Osmotically lyscd rat liver mitochondria have bccn utilized for a study of the biochemical and ultrastructural properties in relation to divalent ion accumulation. Osmotic lysis of mitochondria by suspension and washing in cold, distilled water results in the extraction of about 50 % of the mitochondrial protein, the loss of the outer mitochondrial membrane,, an increase in respiration, and a m...

متن کامل

OSMOTICALLY LYSED RAT LIVER MITOCtIONDRIA Biochemical and Ultrastructural Properties in Relation to Massive Ion Accumulation

Osmotically lyscd rat liver mitochondria have bccn utilized for a study of the biochemical and ultrastructural properties in relation to divalent ion accumulation. Osmotic lysis of mitochondria by suspension and washing in cold, distilled water results in the extraction of about 50 % of the mitochondrial protein, the loss of the outer mitochondrial membrane,, an increase in respiration, and a m...

متن کامل

OSMOTICALLY LYSED RAT LIVER MITOCtIONDRIA Biochemical and Ultrastructural Properties in Relation to Massive Ion Accumulation

Osmotically lyscd rat liver mitochondria have bccn utilized for a study of the biochemical and ultrastructural properties in relation to divalent ion accumulation. Osmotic lysis of mitochondria by suspension and washing in cold, distilled water results in the extraction of about 50 % of the mitochondrial protein, the loss of the outer mitochondrial membrane,, an increase in respiration, and a m...

متن کامل

BIOCHEMICAL AND ULTRASTRUCTURAL PROPERTIES OF OSMOTICALLY LYSED RAT-LIVER MITOCHONDRIA

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